Surface sterilization and culturing
See Bascom-Slack et al., 2012, particularly the supplemental material. This formed the basis for our protocol and data sheet (see Appendix). Briefly, leaf tissue is surface-sterilized in ethanol and commercial bleach followed by incubation on appropriate media (PDA or MEA).
Purification and long-term storage
We have followed established protocol to purify fungi to generate long-term stocks for later analysis (Bascom-Slack et al., 2012 and A. Fatsi – see Appendix).
