Answer: Yes! Experiment Growing tips of fungi emerging from leaf tissue is excised and transplanted to a new culture plate. If culture is pure (by visual inspection), thrice-autoclaved barley seeds are added to the fungi. The fungi overgrow and colonize the barley seeds which are then stored at -70oC. To recover the fungi, a colonized
Project 3) Can we store and recover fungi for long-term storage? Read More »
Endophyte 1.0 (Fall semester- early Spring 2014) ANSWER: YES! We see some morphotypes consistently emerging from Magnolia leaf tissue. Experiment: Our first experiments were exploratory in nature. We adapted published techniques to conditions in our lab (Bascom-Slack et al., 2012 and supplementary material; Appendix). We explored various ways of consistently producing equivalent size pieces of tissue
Project 2) Can we culture any endophytes from Magnolia leaf tissue? Read More »
ANSWER: All kinds of stuff, but very different from those we find in Magnolia. Experiment: MEA (malt extract agar) or PDA (potato dextrose agar) plates were left open for 16-24 hours in the lab, sealed with parafilm and incubated at room temperature for 4-5 days. Results: These fungi are a different population than those
Project 1) What’s in the Air? Read More »
Poster presented by De’Jana Parker, Masters candidate at the 2017 Plant Biology Meeting in Oahu, HI
Endophytes Colonize Read More »
